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![Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Western-Blot-NBP1-06713-img0009.jpg "Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Immunocytochemistry-Immunofluorescence-NBP1-06713-img0006.jpg)
![Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - The A08a neuron receives arbor-specific synaptic inputs. Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. Image using the DyLight 650 form of this antibody (NBP1-06713C).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-BSA-Free-Immunohistochemistry-NBP1-06713-img0010.jpg)
![Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG (TM) and ANTI-FLAG (TM) are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Western-Blot-NBP1-06713-img0001.jpg)
![Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - OLLAS tagged SFO-p41 protein.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Western-Blot-NBP1-06713-img0003.jpg)
![Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Whole-Mount-NBP1-06713-img0005.jpg)
![Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Frozen-NBP1-06713-img0004.jpg)
![Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha-MES-4 antibody and K76 alpha-PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30201), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0008.jpg)
![Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top), nSyb::CD19 (middle) and CD19::Sdc (bottom), with the R17H02 driver (in green). The brain samples were co-immunostained with antibodies against the OLLAS tag (present in ligand) & against the pre-synaptic protein, BRP (in magenta). The ligand proteins are co-localized with or adjacent to BRP (arrows), demonstrating that all ligands are enriched at the presynaptic terminals of the ORNs; CD19::Nrx was also expressed at strong levels in the axon shaft outside of the glomerulus (arrowheads in top panels). Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/32027), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0007.jpg)
![Western Blot: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - Utilization of pMVP for the creation of unique PDX1 vectors. pENTR plasmids containing cDNA for human PDX1 with or without a stop codon (i.e. open) were recombined with pMVP components to generate an assortment of PDX1-expressing (A) adenovirus & (C) expression plasmid vectors. (B) Immunoblot blot analysis of INS1 832/13 cell lysates harvested 48h after treatment with crude adenovirus lysates. For the epitope tagged conditions, note the appearance of the endogenous (lower) & overexpressed (upper) PDX1 bands (top blot). For eGFP blot, the use of P2A adds 23 amino acids to the N-terminal protein (i.e. eGFP). Immunoblot analysis of HEK293 cell lysates 24 h after transfection of expression vectors encoding PDX1 with C-terminal (D) epitope tags, (E) eGFP reporter or fusion, (G) & mCherry reporter or fusion. (F, H) Fluorescence microscopy of the eGFP & mCherry containing conditions analyzed in panels E & G. Visible bands resulting from ‘Uncleaved’ P2A products (red circle •) & degradation products (*) are labeled. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30590691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06713_rat-monoclonal-ollas-epitope-tag-antibody-l2-310202416154624.jpg)
![MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. (A) Volcano plot showing log2 fold change of gene expression between mes-2(RNAi) and wild-type L1 PGCs. The numbers of genes whose expression were up or downregulated in mes-2(RNAi) compared to wild-type L1 PGCs are indicated. Dashed lines mark the significance cutoff of q = 0.05 above which genes were counted as misexpressed. (B) Bar graph showing chromosomal distribution of mes-2(RNAi) upregulated genes. Asterisks indicate significantly more genes than expected (hypergeometric test, p-value<0.001 [**]). (C) Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha -MES-4 antibody and K76 alpha -PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29111977), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06713_rat-monoclonal-ollas-epitope-tag-antibody-l2-immunocytochemistry-immunofluorescence-132202617584118.jpg)
![Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.](http://images.novusbio.com/fullsize/N-OLLAS-Epitope-Tag-Kit-Western-Blot-NBK1-18296-img0003.jpg "Western Blot: N OLLAS Epitope Tag Kit [NBK1-18296] - Western Blot on OLLAS tagged SFO-p41 protein.")
![Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Epitope-Tag-Kit-Western-Blot-NBK1-19033-img0003.jpg "Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Epitope-Tag-Kit-NBK1-19033-img0002.jpg)
![Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07084-img0003.jpg "Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![ND OLLAS/DYKDDDDK Vector [NBA1-07084] - NBA1-07084: Vector Map of pCMV-ND OLLAS](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-NBA1-07084-img0004.jpg)
![Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07085-img0001.jpg "Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-NBA1-07085-img0002.jpg)